Description

The FLuc mRNA will express a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

This mRNA is capped using CleanCap, TriLink's proprietary co-transciptional capping method, which results in the naturally occuring Cap 1 structure with high capping efficiency. It is polyadenylated, modified with 5-methoxyuridine and optimized for mammalian systems. It mimics a fully processed mature mRNA.

Product Details

Catalog NoL-7202

PurityPasses Agarose Gel Mobility

Length1929 nucleotides

Base CompositionFully substituted with 5-Methoxy-U

Concentration1.0 mg/mL

Buffer1 mM Sodium Citrate pH 6.4

Conversion Factor40 µg/OD260

Recommended StorageAt or below -40°C

ApplicationReporter Genes

CapAG Start, Cap 1, CleanCap

Other Name(s)CleanCap®Firefly Luciferase mRNA (5-methoxyuridine)