EpiCypher是一家為表觀遺傳學(xué)和染色質(zhì)生物學(xué)研究提供高質(zhì)量試劑和工具的專業(yè)制造商。EpiCypher能提供全長(zhǎng)重組人SMARCA2重塑酶。SMARCA2是ATP依賴性染色質(zhì)重塑復(fù)合物SWI/SNF的核心ATP酶亞基，可動(dòng)員核小體，在基因轉(zhuǎn)錄調(diào)控、發(fā)育、基因組復(fù)制和修復(fù)中發(fā)揮關(guān)鍵作用[1,2]。SWI/SNF亞基的異常活性與超過(guò)20%的人類癌癥和多種發(fā)育障礙相關(guān)，這使得SMARCA2成為一個(gè)新興的候選治療靶點(diǎn)[3,4]。SMARCA2可用于結(jié)合EpiDyne®-FRET Remodeling Assay Substrate (EpiCypher 16-4201)在HTS兼容藥物發(fā)現(xiàn)研究。

[1] Clapier & Carins Annu. Rev. Biochem. (2009). PMID: 19355820

[2] Sundaramoorthy & Owen-Hughes F1000Research (2020). PMID: 32864100

[3] Kadoch & Crabtree Sci. Adv. (2015). PMID: 26601204

[4] Cenik & Shilatifard Nat. Rev (2021). PMID: 32958894

產(chǎn)品詳情

宿主來(lái)源: Human

表達(dá)系統(tǒng): SF9 cells

保存溫度: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.

運(yùn)輸溫度: Dry ice.

產(chǎn)品形式: SMARCA2 at 0.175 mg/mL in 20.7 µL of 25 mM HEPES pH 7.6, 0.1 mM EDTA, 10% glycerol, 350 mM NaCl, 1 mM DTT, 0.4 mM PMSF, 1 mM benzamidine, 0.02% NP-40, 0.4 mg/mL insulin

驗(yàn)證數(shù)據(jù)

Figure 1: ATP-dependent chromatin remodeling assay EpiDyne®-FRET Chromatin Remodeling Substrate (EpiCypher 16-4201; 20 nM) incubated with SMARCA2 Remodeling Enzyme (concentrations indicated) in 1x SMARCA Remodeling Assay Buffer. Curves denote FRET efficiency/chromatin remodeling.

Figure 2: Protein gel data SMARCA2 Remodeling Enzyme (1 µg) was run on an SDS-PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated.

訂購(gòu)詳情

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